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. 2021 Jun 21;18:140. doi: 10.1186/s12974-021-02183-2

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — NanoString analysis demonstrates significant changes in gene expression with HFD. Male and female mice were fed control (Ctr) or high fat diet (HFD) for 14 weeks. A Hypothalami from 3 mice per group were dissected and the area containing the arcuate nucleus, and the median eminence from the 300-um coronal section was excised and 50 ng RNA used in NanoString analysis. Genes exhibiting significant changes in expression are highlighted with heat-maps. B The anterior portion containing POA and OVLT was excised and used in a separate NanoString neuroinflammation panel. Genes exhibiting significant changes in expression are listed