Fig. 2.

Summary of experiments showing TET-dependent, TDG-independent demethylation of the Il4 locus during Th2 differentiation. A Heat maps depicting the percentage of (5mC + 5hmC)/total C in 64 CpGs in the Il4 locus in wildtype naïve CD4⁺ T cells and Th2 cells after the first and second cycles of differentiation, as determined by BS-seq. Data show the results of thousands of sequencing reads from two or three independent experiments. The numbers of CpGs in each amplicon are shown above the heat maps. B, C Heat maps depicting the percentage of (5mC + 5hmC)/total C in 64 CpGs in the Il4 locus in WT vs. TET iTKO cells (B), or WT vs. TDG iKO cells (C), as determined by BS-seq. Results for Th2 cells after the first and second cycles of differentiation are shown. The data show the results of thousands of sequencing reads from two independent experiments. D Heat maps depicting the percentage of (5fC + 5caC)/total C in 47 CpGs in the Il4 locus in WT vs. TDG iKO cells, as determined by PB-seq. Tdg gene deletion was achieved using Cre-ERT2 (upper) or CD4Cre (lower). Results for Th2 cells after the first cycle of differentiation are shown