Figure 9.

Histological images of the spleen, liver, cervical region and kidney removed for histopathological analysis after necropsy (spleen and kidney with 200× magnification; liver and neck area with 100× magnification). On the cervical region, in the subcutaneous tissue, a mixed inflammatory infiltrate was observed, composed mainly of neutrophils and macrophages, associated with a black granular pigment. Some cells had this pigment on the cytoplasm. These findings showed higher grades of severity on group 5 and 6 (6 h and 24 h, respectively). For the remaining organs, no histological alterations were observed (H&E staining).