FIG 2.

Effect of selected quinolin-4(1H)-imines on mitochondrial membrane potential (ΔΨm) of Leishmania parasites. L. infantum axenic amastigotes (A) and L. infantum (B), L. amazonensis (C), and L. major (D) promastigotes were treated for 45 min with different concentrations of selected quinolin-4(1H)-imines. CCCP, carbonyl cyanide m-chlorophenyl hydrazine, uncoupler of the ΔΨm, was used as the positive control. Graphs represent the mean fold change in treated parasites relative to control cells (treated with DMSO) and standard deviations (error bars) from 3 independent experiments. Statistical significance was determined using 2-way analysis of variance (ANOVA) with Sidak’s multiple-comparison test. *, P < 0.05; **, P < 0.005; ***, P < 0.0005; ****, P < 0.0001.