FIG 3.

Analysis of ROS production in Leishmania parasites after the addition of selected quinolin-4(1H)-imines. L. infantum axenic amastigotes (A) and L. infantum (B), L. amazonensis (C), and L. major (D) promastigotes were treated with different concentrations of the selected quinolin-4(1H)-imines for 45 min. AA, antimycin A, was used as the positive control for ROS production. Graphs show the mean values and standard deviations (error bars) from 3 independent experiments. Statistical significance was determined using 2-way ANOVA with Sidak’s multiple-comparison test. *, P < 0.05; **, P < 0.005; ***, P < 0.0005; ****, P < 0.0001.