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. 2021 Jun 7;12:667433. doi: 10.3389/fphar.2021.667433

FIGURE 5.

FIGURE 5

Effects of over-expression and knockdown of HIF-1α on relative markers in HEK293T cells. Cells were divided into following groups: conrtol, negative control (NC), HIF-1α-overexpression group (OE) and HIF-1α-knockdown group (KD). (A) Effects of over-expression of HIF-1α on mRNA levels of HIF-1α, PKM2, CTGF, TGF-β1, and TNF-α were examined by using qPCR. (B) Effects of over-expression of HIF-1α on immunofluorescence staining of HIF-1α and PKM2. (C) Effects of over-expression of HIF-1α on proteins of HIF-1α, PKM2, GLUT1, LDH-A, and IL6 in HEK293T cells were examined by using western blotting. (D) Effects of knockdown of HIF-1α on proteins of HIF-1α, PKM2, GLUT1, LDH-A, and IL6 in HEK293T cells were examined by using western blotting. (E) Effects of knockdown of HIF-1α on mRNA levels of HIF-1α, PKM2, CTGF, TGF-β1, and TNF-α in HEK293T cells were examined by using qPCR. * p < 0.05 and ** p < 0.01.