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Binding mode of the FHNA series in the CHIKV nsP1 secondary binding pocket. (A) The FHNA series is suggested to bind to a secondary binding pocket (pocket 2) in the RAMBO domain of CHIKV nsP1 (PDB code 6Z0V) outside of the main binding pocket. (B) The defined binding pocket is flanked by residues G230n and K299n–1. FHNA and FHA have similar binding modes that are not shared by the inactive analogue FMA.
