FIG 1.

Effects of single mutations on the amount of ciprofloxacin required to inhibit growth of P. aeruginosa PAO1. MICs of ciprofloxacin for parental strain PAO1 and isogenic mutants are shown. Each point represents a biological replicate. The breakpoint for ciprofloxacin susceptibility is ≤0.5 mg/liter and intermediate resistance is 1 mg/liter (clsi.org). MICs for the gyrA T83I mutant are in yellow (intermediate resistance), and MICs of strains carrying the other mutations are in turquoise (susceptible).