FIG 4.

Effects of rnfC H87P on ciprofloxacin MIC. P. aeruginosa PAO1 was engineered to contain mutations in gyrA, rnfC, nfxB, and parC or parE. CK3 is an experimentally evolved mutant of strain PAO1 containing gyrA, rnfC, and nfxB mutations. The wild-type rnfC allele was engineered into CK3 with two independent derivatives being analyzed. Genotypes of mutants are shown, where ✓ denotes the presence of mutation. (A) and (B) are independently engineered biological replicates. Each point represents one replicate of MIC testing. Orange and red denote MICs of 2 mg/liter and >2 mg/liter, respectively.