TABLE 1.
List of amino acid variations searched in clinical isolates of P. aeruginosa to predict the ciprofloxacin resistance
| Protein | Amino acid variation | Reference for presence in clinical isolates or experimentally evolved mutants | Reference for quantification of resistanceb | PROVEAN valuea |
|---|---|---|---|---|
| G81C | 24 | - | −8.7 | |
| G81D | 23 | - | −6.8 | |
| GyrA | T83I | This study, 15, 24 | This study, 15 | −3.7 |
| Y86N | 23 | - | −8.7 | |
| D87N | This study, 15, 24 | This study, 15, 22 | −4.6 | |
| D87G | 23 | This study, 22 | −6.6 | |
| Q106L | 23 | - | −6.8 | |
| E153K | 21 | - | −3.8 | |
| S612L | 24 | - | −5.3 | |
| GyrB | S466Y | This study, 15 | 15 | −5.1 |
| S466F | This study, 15, 16 | 15 | −5.1 | |
| P749S | This study | - | −5.9 | |
| NfxB | P22Q | This study | - | −6.7 |
| A30V | This study | - | −3.9 | |
| A38G | This study | - | −3.8 | |
| T39P | 24 | This study, 22 | −5.6 | |
| R42C | This study, 76 | - | −7.5 | |
| E75G | This study | - | −3.8 | |
| R82L | This study, 77 | - | −4.5 | |
| H87R | 76 | - | −4.8 | |
| ParC | S87L | This study, 24 | This study | −5.9 |
| S87W | 15 | 15 | −6.9 | |
| ParE | M437I | 15 | - | −2.9 |
| P438S | This study, 23 | - | −7.9 | |
| S457C | This study, 23 | - | −4.9 | |
| V460G | 24 | This study | −6.9 | |
| A473V | This study, 78 | - | −2.4 | |
| Plasmid mediated protein | CrpP | This study, 29 | - | - |
a
PROVEAN analysis was carried out as described in reference 24. Variants with PROVEAN values equal to or below −2.5 are likely to be function-altering variants.
b
The symbol - indicates not tested.