FIG 1.

FC-10696 inhibits budding of mVP40 VLPs in a dose-dependent manner. HEK293T cells were transfected with mVP40 in the presence of DMSO alone, compound 1, or the indicated concentrations of compound FC-10696. mVP40 was detected in cell lysates and VLPs by Western blotting, and mVP40 levels in VLPs were quantified using NIH ImageJ software. (B) Quantitative results and statistical significance (one-way analysis of variance [ANOVA]) are shown for 3 independent mVP40 VLP budding assays. **, P < 0.01.