TABLE 2.

Impact of a freeze-thaw cycle on exebacase MICs against 25 S. aureus isolates

Mediuma	Freeze-thaw	MIC (μg/ml)			Trailing
		50%	90%	Range	effect
CAMHB	No	32	64	2–128	Yes
	Yes	128	256	32–>256	Yes
CAMHB, 25% horse serum	No	0.5	2	0.125–2	No
	Yes	2	2	0.5–8	No
CAMHB, 0.5 mM DTT	No	128	128	8–256	Yes
	Yes	128	256	64–>256	Yes
CAMHB, 25% horse serum, 0.5 mM DTT	No	0.5	1	0.25–2	No
	Yes	0.5	1	0.25–2	No

^aMedium was prepared using reagents from the following sources: CAMHB (BBL Mueller-Hinton broth II, cation adjusted), Becton Dickinson, catalog number 212322; horse serum (donor herd), Sigma-Aldrich, catalog number H1270; and DTT, Sigma-Aldrich, catalog number 646563.