Figure 1.

Expression of PD-1, TIGIT, CD73 and CD39 on CD8⁺ and conCD4⁺ and regCD4⁺ T cells in pAML and HDs

The expression of PD-1, TIGIT, CD73 and CD39 was analyzed in peripheral blood (PB) from healthy donors (HD, white circles, n = 12) and from patients with newly diagnosed AML (pAML, PB, black circles, n = 20) as well as in bone marrow aspirates (BM, black triangles, n = 24). The gating strategy is shown in Supplemental Figure 3. (A) Representative flow data showing the expression levels of PD-1, TIGIT, CD73, and CD39 on CD8⁺ T cells in the PB from HDs (gray) and PB from patients with pAML (red). (B) Frequencies of PD-1, TIGIT, CD73, CD39 expression on indicated T-cell subsets were shown in the PB from HDs and patients with pAML and in the BM. P values were obtained by the ANOVA and Kruskal-Wallis test. *P < .05, **P < .01, ***P < .001.