Figure 2.

Increased expression of PD-1 and CD39 on TIGIT⁺CD73⁻CD8⁺ T cells in pAML

Flow cytometric analysis of the co-expression of PD-1 and CD39 with TIGIT and CD73 on CD8⁺ T cells was performed. (A) Summary data of the frequency of TIGIT^± and CD73^± CD8⁺ T-cell subsets in PB from healthy donors (HD PB, white circles, n = 12) and patients with newly diagnosed AML (pAML PB, black circles, n = 18), and bone marrow aspirates from pAML patients (pAML BM, black triangles, n = 24). (B) Summary data illustrating the frequency of PD-1⁺ and CD39⁺ cells on CD8⁺ T cells expressing TIGIT and/or CD73 from PB of HDs and pAML patients, and BM aspirates from pAML patients. (C) tSNE analysis showing a heat map of total CD8⁺ T cells from PB samples of five patients with pAML (left graph) and the position of CD8⁺ T-cell differentiation stages (blue = naïve; yellow = central memory; green = effector memory and red = terminal differentiated effector memory CD8⁺ T cells) on the tSNE map. (D) Distribution of TIGIT⁺CD73⁻ cells (green overlay), PD-1⁺ cells (red overlay), and CD39⁺ cells (violet overlay) on the tSNE map of CD8⁺ T cells (gray). P values were obtained by ANOVA and Kruskal-Wallis test. *P < .05, **P < .01, ***P < .001.