Table 1.
Summary of Liquid Biopsy Samples With Detectable ctDNA From pHGG/DIPG Patients
| Patient ID | ddpcr Assay ID | SAMPLE type | Volume for ctDNA | VAF | Mutant Droplets | Wild-type Droplets | cfDNA Mutant ng/mL | cfDNA Wild-Type ng/mL |
|---|---|---|---|---|---|---|---|---|
| 013-T | H3F3A -K27M | Plasma | 2.7 | 2.451 | 10 | 398 | 0.028 | 1.131 |
| 045-T | TP53-R282W | CSF | 5 | 49.343 | 3945 | 4050 | 271.913 | 279.585 |
| 045-T | TP53-R282W | Plasma | 5 | 0.118 | 3 | 2540 | 0.021 | 18.313 |
| 054-T | H3F3A-G34R | Plasma | 5 | 0.468 | 2 | 425 | 0.007 | 1.561 |
| 106-T | IDH1-R132H | Plasma | 3.5 | 0.335 | 11 | 3272 | 0.029 | 8.844 |
| 120-T | H3F3A-G34R | Plasma | 3 | 1.143 | 2 | 173 | 0.005 | 0.422 |
| 131-T | H3F3A -K27M | Cyst | 38.5 | 42.68 | 10944 | 14698 | 367.594 | 541.671 |
| 131-T | H3F3A -K27M | Plasma | 2 | 0.85 | 3 | 350 | 0.009 | 1.048 |
| 15–3381 | H3F3A -K27M | CSF | 1 | 3.646 | 7 | 185 | 0.044 | 1.177 |
| 16120B | PIK3CA-E542K | Serum | 2 | 0.072 | 7 | 9674 | 0.291 | 472.795 |
| ICR-B134 | H3F3A -K27M | Serum | 1.3 | 0.39 | 2 | 511 | 0.015 | 3.934 |
| ICR-B276 | PIK3CA-H1047R | Plasma | 2 | 0.116 | 2 | 1727 | 0.006 | 5.098 |
| ICR-CXJ-026 | TP53-C238Y | CSF | 2.5 | 2.073 | 4 | 189 | 0.013 | 0.633 |
| ICR-CXJ-028 | ACVR1 G328V | CSF | 1.8 | 35.737 | 446 | 802 | 1.472 | 2.662 |
| I-16–3200 | H3F3A -K27M | CSF | 0.7 | 0.051 | 3 | 5916 | 0.071 | 159.274 |
| I-16–855 | H3F3A -K27M | CSF | 1.5 | 1.149 | 9 | 774 | 0.054 | 4.703 |
Shown are the mutations assessed by ddPCR, the biological source material, volume used for the ddPCR assay, variant allele frequency (VAF), number of mutant and wild-type droplets as well as concentration of mutant and wild-type cfDNA.