Figure 5.

Cannabinoid-mediated changes in bacterial composition are inconsequential to DSS progression. [A] PCOA plot displaying the bacterial community clustering from indicated mice on Day 14 of DSS colitis [n = 5]. [B] Caecal SCFA quantification of indicated mice [n = 5]. Mice were given antibiotics [ABX] in their drinking water for 4 weeks before antibiotic-free water was returned, mice were placed in the cages of donor faecal transfer [FT] mice containing their used bedding. Three days after cessation of antibiotics, mice were given daily faecal transfers from stool of indicated donor mice. DSS [2%] was administered in the drinking water after 3 days of faecal transfers; and [C] body weight was recorded throughout the study [n = 7–8 per group]. Data are presented as mean ± standard error of the mean [SEM]. Faecal transfer-DSS experiment was repeated three times. Data presented are from a single representative experiment. NS, not significant. 16S sequencing results and SCFA quantification are from one representative DSS experiment that was repeated four times.