Figure 6.

Haematopoietic and non-haematopoietic cells contribute to THC-mediated colitis protection. WT mice were myelo-ablated via two doses of 600 cGy separated by 3 h, then immune reconstitution was accomplished by transfer of bone marrow cells from WT [WT → WT] or Cnr2-/-, CB2 knockout [CB2 → WT] mice. [A] Body weight, and [B] colon lengths from indicated groups [n = 4–5]. [C] Flow cytometric analysis of CD4+ IL-17A+ and IFNγ+ cells in the colonic lamina propria [n = 4–5]. Each symbol represents an individual mouse. Data are from one experiment representative of two independent experiments and presented as mean ± standard error of the mean [SEM]. NS, not significant; *p <0.05; **p <0.01, ***p <0.001, ****p <0.0001 by two-way ANOVA with Tukey’s multiple comparisons test.