Table 3.
List of reagents.
| Fluorescent antibody | Clone | Identifier | Source |
|---|---|---|---|
| APC/Cy7 anti-mouse CD45 | 30-F11 | 103115 | Biolegend |
| Brilliant Violet 785™ anti-mouse CD4 | GK1.5 | 100453 | Biolegend |
| Brilliant Violet 421™ anti-mouse FOXP3 | MF-14 | 126419 | Biolegend |
| Mouse Neuropilin-1 Alexa Fluor® 488-conjugated | 761704 | FAB59941G | RandD Systems |
| Alexa Fluor® 700 anti-mouse/human CD11b | M1/70 | 101222 | Biolegend |
| Alexa Fluor® 700 anti-mouse lineage cocktail with isotype Ctrl | 17A2/RB6-8C5/RA3-6B2/Ter-119/M1/70 | 133313 | Biolegend |
| Brilliant Violet 605™ anti-mouse CD11c | N418 | 117334 | Biolegend |
| PerCP/Cyanine5.5 anti-mouse CD103 | 2E7 | 121416 | Biolegend |
| Brilliant Violet 421™ anti-mouse F4/80 | BM8 | 123137 | Biolegend |
| PE/Dazzle™ 594 anti-mouse CD117 [c-Kit] | 2B8 | 105834 | Biolegend |
| PerCP anti-mouse CD8a | 53–6.7 | 100732 | Biolegend |
| PE anti-mouse FcεRIα | MAR-1 | 134308 | Biolegend |
| PE anti-mouse Ly-6G/Ly-6C [Gr-1] | RB6-8C5 | 108407 | Biolegend |
| Alexa Fluor® 647 anti-mouse I-A/I-E | M5/114.15.2 | 107617 | Biolegend |
| PE mouse anti-mouse RORγt | Q31-378 | 562607 | BD Biosciences |
| Brilliant Violet 605™ anti-T-bet | 4B10 | 644817 | Biolegend |
| PE/Dazzle™ 594 anti-mouse/human Helios | 22F6 | 137232 | Biolegend |
| PE/Dazzle™ 594 anti-mouse CD196 [CCR6] | 29-2L17 | 129822 | Biolegend |
| Brilliant Violet 510™ anti-mouse CD335 [NKp46] | 29A1.4 | 137623 | Biolegend |
| PE/Dazzle™ 594 anti-mouse CD197 [CCR7] | 4B12 | 120122 | Biolegend |
| BV786 rat anti-mouse CD103 | M290 | 564322 | BD Biosciences |
| BB700 rat anti-mouse I-A/I-E | M5/114.15.2 | 746197 | BD Biosciences |
| TruStain FcX™ [anti-mouse CD16/32] | 93 | 101320 | Biolegend |
| or Reagent | Dose/route of administration | Catalogue number | Source |
| Dextran sulphate sodium salt [DSS], colitis grade [36,000–50,000 MW] | Drinking water [2% wt/vol] | 0216011080 | MPBIO |
| Azoxymethane | 10 mg/kg, i.p. | 25843-45-2 | MPBIO |
| Picrylsulphonic acid solution [TNBS] | 1 mg, intrarectal | P2297 | Millipore-Sigma |
| ∆9-tetrahydrocannabinol solution | 10 mg/kg, oral gavage | T2386 | Millipore-Sigma |
| ∆9-THC | 10 mg/kg, oral gavage | 12068 | Cayman Chemical |
| Cannabidiol | 10 mg/kg, oral gavage | 90080 | Cayman Chemical |
| SR 144528 | 10 µM | 5039 | Tocris |
| AM 251 | 10 µM | 1117 | Tocris |
| Recombinant mouse GM-CSF [carrier-free] | 20 ng/mL | 576304 | Biolegend |
| Recombinant mouse IL-4 [carrier-free] | 10 ng/mL | 574304 | Biolegend |
| Recombinant mouse IFN-γ [carrier-free] | 40 ng/mL | 575304 | Biolegend |
| Recombinant human IFN-γ [carrier-free] | 40 ng/mL | 570204 | Biolegend |
| Purified anti-mouse CD3ε [clone 145-2C11] | 1 µg/mL | 100301 | Biolegend |
| Ultra-LEAF™ purified anti-mouse CD28 [clone 37.51] | 4 µg/mL | 102115 | Biolegend |
| Recombinant mouse IL-6 [Animal-Free] | 30 ng/mL | 715202 | Biolegend |
| Recombinant mouse IL-23 [carrier-free] | 20 ng/mL | 589002 | Biolegend |
| Recombinant mouse IL-1β [carrier-free] | 10 ng/mL | 575102 | Biolegend |
| 6-Formylindolo[3,2-b]carbazole [FICZ] | 400 nM | SML1489 | Millipore-Sigma |
| TGF-beta 1, 2, 3 | 5 µg/mL | MAB1835R | RandD Systems |
Wt/vol, weight/volume. i.p., intraperitoneal.