Table 4.

qRT-PCR primers.

Gene name	Accession number	Primer	Sequence 5’ → 3’
mMUC2	NM_023566	Forward	CTACCATTACCACCACTAC
		Reverse	GTCTCTCGATCACCACCATTT
mMUC5ac	NM O88715	Forward	CTGTAACACCCAGTGTCCTAAG
		Reverse	AGGCTGGTAGAAGTAGGTAGAG
m18S	NR_003278	Forward	CGTCGTAGTTCCGACCATAAA
		Reverse	TTTCAGCTTTGCAACCATACTC
mβ-actin	NM_007393	Forward	GGCTGTATTCCCCTCCATCG
		Reverse	CCAGTTGGTAACAATGCCATGT
mβ-Defensin 1	NM_007843	Forward	CACAGGCTTCCTGGGATATAAA
		Reverse	CGCTCTGGTTGGACAACTTA
mβ-Defensin 3	NM_013756	Forward	TTGAGGAAAGGAGGCAGATG
		Reverse	CGGGATCTTGGTCTTCTCTATTT
mZO-1	NM_009386	Forward	GCCGCTAAGAGCACAGCAA
		Reverse	TCCCCACTCTGAAAATGAGGA
mClaudin18	NM_001194921	Forward	TGGGTTTTGTGGTGTCACTG
		Reverse	GGTAGTTGAATACAGCGGTCAC
mOccludin	NM_001360536	Forward	TTGAAAGTCCACCTCCTTACAGA
		Reverse	CCGGATAAAAAGAGTACGCTGG
mLyz-1	NM_013590	Forward	CCTCCAAGTAACAGGACTTCAG
		Reverse	CTGACTGACAAGGGAGACTTTG
mLyz-2	NM_017372	Forward	AGTTCTTCAGCCAGGAAGTG
		Reverse	CCAAGATCAACTGGTCTCCTATAA
hB2M	NM_004048	Forward	GAGGCTATCCAGCGTACTCCA
		Reverse	CGGCAGGCATACTCATCTTTT
hRPLPO	NM_053275	Forward	CCATTCTATCATCAACGGGTACAA
		Reverse	TCAGCAAGTGGGAAGGTGTAATC
hDEFB1	NM_005218	Forward	GGTGGGTCAAAATGTGTGAGT
		Reverse	GCTGTGGTAGGTCAGGCTTC
hDEFB103A	NM_001081551.3	Forward	TGCTCTTCCTGTTTTTGGTGC
		Reverse	TGCCGATCTGTTCCTCCTTT
hZO-1	NM_001301025	Forward	CGGTCCTCTGAGCCTGTAAG
		Reverse	GGATCTACATGCGACGACAA
hMUC2	NM_002457	Forward	CACCTGTGCCCTGGAAGGC
		Reverse	CGGTCACGTGGGGCAGGTTC
hMUC5AC	NM_017511	Forward	CGGGTCCACGAGGAGACGGT
		Reverse	GCTTCTGCAGCCAGGCACGA
hGAPDH	NM_002046	Forward	GAAGGTCGGAGTCAACGGATT
		Reverse	CGCTCCTGGAAGATGGTGAT