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. 2021 Jun 3;12:681380. doi: 10.3389/fimmu.2021.681380

Figure 3.

Figure 3

RAE-1γMCMV-SIINFEKL has superior priming capacity and generates phenotypically distinct memory CD8 T cells. (A) 104 naive OT-1 cells were transferred to C57BL/6J mice one day prior to immunization with MCMV-SIINFEKL or RAE-1γMCMV-SIINFEKL (n=4-5). (B) OT-1 frequency (blood) at indicated time points post-immunization. (C) Absolute numbers of OT-1 cells at day 7 post-immunization in the spleen. (D) Kinetics of TCF1+ and KLRG1+ populations were followed in blood over time. (E) The phenotype of memory OT-1 cells primed with indicated viruses at day 37 post-immunization shown as the percentage of TCF1+, CD62L+, CD27+, CD127+ OT-1 cells, and MFI of Eomes on OT-1 cells. (F) Expression of indicated molecules on TCF1+ and TCF1- cells. Data are from a single experiment representative of two independent experiments. Data are represented as mean ± SEM and statistical significance as *p < 0.05, **p < 0.01, ****p<0,0001. Statistical significance was determined using unpaired Student t-test.