FIG 2.
Standard operating procedure for evaluation of the biological and technical variability in the identification of CPK isolates followed by the Complejo Hospitalario Universitario de A Coruña (CHUAC), Hospital General Universitario Gregorio Marañón (HGUGM), and Hospital Universitario Puerta del Mar (HUPM). The HGUGM and HUPM only evaluated the methods associated with least imprecision to complete the reproducibility phase. The CVs associated with each step of the procedure are illustrated in the figure. CV, level of imprecision associated with the extraction (CVEXT) or the MALDI-TOF MS process (CVMALDI) and total or technical imprecision (CVT); BV, biological variation of isolates; BVINTER, interindividual BV; BVINTRA, intraindividual BV.