Table 6.
Expression of liver genes in beef cows that received diets containing supplemental INR (n = 95) or AAC (n = 95) during gestation1,2
| Item | INR | AAC | SEM | P-value |
|---|---|---|---|---|
| CUT | ||||
| Initial (day −30) | 1.86 | 1.43 | 0.21 | 0.18 |
| Pre-calving (day 97) | 1.67 | 1.70 | 0.08 | 0.82 |
| Calving | 1.95 | 1.75 | 0.11 | 0.19 |
| MT | ||||
| Initial (day −30) | 3.39 | 2.38 | 0.73 | 0.36 |
| Pre-calving (day 97) | 26.1 | 22.8 | 3.8 | 0.54 |
| Calving | 36.4 | 65.6 | 11.6 | 0.04 |
| SOD | ||||
| Initial (day −30) | 2.29 | 2.76 | 0.27 | 0.24 |
| Pre-calving (day 97) | 1.99 | 2.11 | 0.13 | 0.51 |
| Calving | 2.02 | 2.04 | 0.13 | 0.90 |
1INR and AAC cows received the same amount of supplemental Co, Cu, Mn, and Zn from sulfate sources or Availa 4 (Zinpro Corporation, Eden Prairie, MN). Cows were assigned to the experiment at 117 ± 2 d of gestation (day 0).
2Liver samples were collected via needle biopsy (Arthington and Corah, 1995) before the beginning of the experiment (day −30; initial), on day 97 (late gestation), and upon calving. Values are expressed as relative fold change compared within CT of reference genes analyzed within the same sample (Ocón-Grove et al., 2008). Samples were collected from 30 cows of each treatment randomly selected on each sampling day.