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. Author manuscript; available in PMC: 2021 Nov 3.
Published in final edited form as: Cell Metab. 2020 Oct 8;32(5):726–735.e5. doi: 10.1016/j.cmet.2020.09.008

Figure 2. HFD feeding induces translocation of PKCθ and PKCε but is unassociated with alterations in pPDH/PDH, mitochondrial metabolites or expression of key oxidative enzymes.

Figure 2.

A. Phosphorylation of muscle PDH Serine293, Serine232, and Serine300 is unchanged in HFD-fed insulin resistant rats compared to regular chow fed rats in both basal and clamped states. B. Muscle concentrations of mitochondrial metabolites are unchanged in muscles of HFD-fed insulin resistant rats. C. Expression of key oxidative enzymes PDH, CPT1b, and CrAT are unchanged in muscles of insulin-resistant rats. D. Insulin-resistant rats have increased PKCθ and PKCε translocation (n=5 for each group). All data measured in quadriceps muscle. Data are represented as mean ± SEM.