KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| PDH-E1α | Abcam | CAT#AB110330 |
| Phospho-PDH (s293) | Cell Signaling | CAT#31866S |
| Phospho-PDH (s232) | Millipore | CAT#AP1063 |
| Phospho-PDH (s300) | Sigma Aldrich | CAT#ABS2082 |
| CPT1b | Abcam | CAT#AB134988 |
| CrAT | ProteinTech | CAT#15170–1-AP |
| PKCθ | Cell Signaling | CAT#2059 |
| IRS-1 | Cell Signaling | CAT#2390 |
| AKT | Cell Signaling | CAT#2920 |
| Phospho-AKT (s473) | Cell Signaling | CAT#4060 |
| Insulin Receptor β | Cell Signaling | CAT#3025 |
| Phospho-IRK (Y1162) | Cell Signaling | CAT#3918 |
| GAPDH | Cell Signaling | CAT#5174S |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Regular insulin | Sigma Aldrich | CAT#NDC 0002–8215-01 |
| 20% glucose | Hospira | CAT #NDC 0409–7935-19 |
| [1,2,3,4,5,6-13C6] glucose | Cambridge Isotopes | CAT#CLM-1396 |
| 2-[1-14C]-Deoxy-D-glucose | Perkin Elmer | CAT#NEC495A001MC |
| [1-13C] D-glucose | Cambridge Isotopes | CAT#CLM-420-PK |
| [1-13C] sodium acetate | Cambridge Isotopes | CAT#CLM-156-PK |
| Intralipid 20% emulsion | Baxter Inc. | CAT#2B6061 |
| Critical Commercial Assays | ||
| HR Series NEFA-HR(2) | Wako | CAT#999–34691 |
| HR Series NEFA-HR(2) | Wako | CAT#995–34791 |
| HR Series NEFA-HR(2) | Wako | CAT#991–34891 |
| HR Series NEFA-HR(2) | Wako | CAT#993–35191 |
| Glucose-6-phosphate Assay Kit | Sigma Aldrich | CAT#MAK014–1KT |
| Experimental Models: Organisms/Strains | ||
| Sprague-Dawley rats | Charles River | Strain code: 400 |
| Other | ||
| Regular chow diet | Harlan Teklad | CAT#2018 |
| High-fat diet | Dyets | CAT#112245 |