Table 2.
Comparison of automated [18F]FDOPA synthesis performance in microscale (at the higher activity level tested) and macroscale. All values are averages ± standard deviations for the indicated number of replicates. Abbreviations: EOS = at end of synthesis
| This work (microdroplet reactor) (n=3) | Kuik et al. (conventional synthesizer) (n=?) | Collins et al. (conventional synthesizer) (n=3) | |
|---|---|---|---|
| Overall synthesis time (min) | ~ 31 | ~ 117 | ~ 71 |
| Precursor amount (μmol [mg]) | 0.12 [0.11] | 13.4 [12] | 16.8 [15] |
| Reaction volume (μL) | 10 | 750 | 1000 |
| Starting activity (GBq) | ~0.37 | Not reported | ~15 |
| Isolated RCY (%, decay corrected) | 4.7 ± 1.0 | 14 ± 4 | 4.5 ± 1.3 |
| Isolated RCY (%, non-decay corrected) | 3.9 ± 1.8 | 6.7 ± 1.9 | 2.9 ± 0.8 |
| Activity yield, EOS (GBq) | ~0.015 | Not reported | ~0.43 |
| Molar activity, EOS (GBq/μmol) | >75 | 30–33 | 35 ± 4 |
| Solvent volume for synthesis (mL) | 0.17 | 6.35 | 13.2 |
| Mobile phase volume for HPLC purification (mL) | 9.3 | 32 | 65 |
| Estimated mobile phase volume for HPLC column equilibration and cleaning (mL) | 48 | 240 | 240 |