Table 7.
The comparison of the PLLA AF and RF influencing PC12 cell differentiation
| PLLA AF | PLLA RF | |
|---|---|---|
| Metabolic pathways important to neuronal differentiation | 6 mutual, 3 specific | 6 mutual, 1 specific |
| Relevant differential metabolites of important metabolic pathways | 14 mutual, 5 specific | 14 mutual, 1 specific |
| Critical metabolic pathways | 2 mutual | |
| ① Phenylalanine, tyrosine, and tryptophan biosynthesis pathway | ||
| ② Phenylalanine metabolism pathway | ||
| Critical metabolites | ①phenylalanine ↓ | ①phenylalanine ↑ |
| ②phenylpyruvic acid ↓ | ②phenylpyruvic acid ↑ | |
| ③tyrosine ↓ | ③tyrosine ↓ | |
| ④22-phenylacetamide ↑ | ||
| Differential expression of the neuronal differentiation marker gene and the genes relevant to neurite outgrowth | High | Low |
| Differential expression of the genes that regulated critical metabolites in cell differentiation | High | Low |
| Cell proliferation rate | Low | High |
| Neurite length (beneficial for cell differentiation) | Long | Short |
| Number of neurites (less effective for cell differentiation) | Small number | Large number |
| Impact on cells | More beneficial to cell differentiation | Less effective for cell differentiation |
↑ represents increased concentration. ↓ represents decreased concentration.