Table 2.
Company | Platform | Run Time | Maximum Output | Maximum Read Length | Reads per run |
---|---|---|---|---|---|
Illumina Inc. | MiSeq | 4 – 55 hrs | 15 Gb | 2 × 300bp | 25 M/ per lane |
NextSeq | 12–30 hrs | 120 Gb | 2 × 150bp | 400 M / per lane | |
HiSeq 3000 | < 1 – 3.5 days | 750 Gb | 2 × 150bp | 2.5 B /per lane | |
HiSeq 4000 | < 1 – 3.5 days | 1,5 Tb | 2 × 150bp | 5 B / per lane | |
HiSeq × Series | < 3 days | 1,8 Tb | 2 × 150bp | 6 B /per flow cell | |
NovaSeq 6000 | ~13 – 38 hrs | N/A | 2 × 250bp | 10 B/per lane | |
Pacific Biosciences Inc | PacBio RS II | 0.5 – 4 hrs | 1Gb | ~10 – 15 kb | 50 – 80 k |
Life Technologies Corp. | Ion GeneStudio S5 | 4.5 –19 hrs | 15 Gb | 200 – 600 bp | 2 – 130M |
Ion GeneStudio S5 Plus | 3 – 20 hrs | 30 Gb | 200 – 600 bp | 2 – 130M | |
Ion GeneStudio S5 Prime | 3–10 hrs | 50 Gb | 200 – 600 bp | 2 – 130M | |
Sequencing by Oligo Ligation Detection | SOLiD 5500 W | 10 days | 120 Gb | 2 × 50bp | 1.2 B |
SOLiD 5500×1 W | 10 days | 240 Gb | 2 × 50bp | 2.4 B | |
Roche Inc. | 454 GS FLX+ | 10–23 hrs | 450 – 700 Mb | Up to 1 kb | 1M |
454 GS Jr | 10 hrs | 35 Mb | 400 bp | 100 k | |
Oxford Nanopore | Flongle | 1 min – 16 hrs | 2 Gb | >2Mb | 126 channels |
MinlON | 1 min – 48 hrs | 50 Gb | 512 channels | ||
GridlON Mk1 | 1 min – 48 hrs | 250 Gb | 512×5 channels | ||
PromethlON 24 | 1 min – 72 hrs | 5.2 Tb | 24×3000 channels | ||
PromethlON 48 | 1 min – 72 hrs | 10.5 Tb | 48×3000 channels |
Abbreviations Mb, Gb and Tb correspond to Megabytes, Gigabytes and Terabytes, respectively (for comparison, the human genome has 3×109 bp or 3 Gb). Abbreviations hrs corresponds to hours, bp to base pair, kb to kilobase, M and B to millions and billions.