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. Author manuscript; available in PMC: 2021 Jun 22.
Published in final edited form as: Phys Chem Chem Phys. 2020 Jan 22;22(12):6492–6506. doi: 10.1039/c9cp05912a

Table 2.

Technical specifications of sequencing products.

Company Platform Run Time Maximum Output Maximum Read Length Reads per run
Illumina Inc. MiSeq 4 – 55 hrs 15 Gb 2 × 300bp 25 M/ per lane
NextSeq 12–30 hrs 120 Gb 2 × 150bp 400 M / per lane
HiSeq 3000 < 1 – 3.5 days 750 Gb 2 × 150bp 2.5 B /per lane
HiSeq 4000 < 1 – 3.5 days 1,5 Tb 2 × 150bp 5 B / per lane
HiSeq × Series < 3 days 1,8 Tb 2 × 150bp 6 B /per flow cell
NovaSeq 6000 ~13 – 38 hrs N/A 2 × 250bp 10 B/per lane
Pacific Biosciences Inc PacBio RS II 0.5 – 4 hrs 1Gb ~10 – 15 kb 50 – 80 k
Life Technologies Corp. Ion GeneStudio S5 4.5 –19 hrs 15 Gb 200 – 600 bp 2 – 130M
Ion GeneStudio S5 Plus 3 – 20 hrs 30 Gb 200 – 600 bp 2 – 130M
Ion GeneStudio S5 Prime 3–10 hrs 50 Gb 200 – 600 bp 2 – 130M
Sequencing by Oligo Ligation Detection SOLiD 5500 W 10 days 120 Gb 2 × 50bp 1.2 B
SOLiD 5500×1 W 10 days 240 Gb 2 × 50bp 2.4 B
Roche Inc. 454 GS FLX+ 10–23 hrs 450 – 700 Mb Up to 1 kb 1M
454 GS Jr 10 hrs 35 Mb 400 bp 100 k
Oxford Nanopore Flongle 1 min – 16 hrs 2 Gb >2Mb 126 channels
MinlON 1 min – 48 hrs 50 Gb 512 channels
GridlON Mk1 1 min – 48 hrs 250 Gb 512×5 channels
PromethlON 24 1 min – 72 hrs 5.2 Tb 24×3000 channels
PromethlON 48 1 min – 72 hrs 10.5 Tb 48×3000 channels

Abbreviations Mb, Gb and Tb correspond to Megabytes, Gigabytes and Terabytes, respectively (for comparison, the human genome has 3×109 bp or 3 Gb). Abbreviations hrs corresponds to hours, bp to base pair, kb to kilobase, M and B to millions and billions.