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. 2021 Jun 8;11:642134. doi: 10.3389/fonc.2021.642134

Figure 1.

Figure 1

NHWD-870 inhibits the proliferation and induces the apoptosis of human osteosarcoma cells in vitro. (A) 143B, HOS, U2OS, and SJSA1 cells were seeded at 5000 cells per well and treated with NHWD-870 at the indicated concentration for 24 or 48 hours. Cell activity was measured by CCK8 . (****P < 0.0001). (B) Colony formation was used to evaluate the inhibitory effects of NHWD-870 on osteosarcoma cell proliferation. (C) The statistical results of the osteosarcoma colony formation test showed that NHWD-870 significantly reduced the colony formation ability of osteosarcoma cells . (****P < 0.0001). (D) Cell apoptosis was analyzed using Annexin V‐FITC/PI flow cytometry. After treatment with the indicated concentration of NHWD-870 for 24 h, Annexin V‐FITC/PI staining was used for flow cytometry analysis of cell apoptosis. Data analysis was performed using Flow Jo software. (E) The FACS statistics and the percentages of apoptotic cells in the indicated cell populations are shown in the histogram and expressed as the mean ± SD of three independent experiments. (**P < 0.01; and ***P < 0.001, and ****P < 0.0001).