Skip to main content
. Author manuscript; available in PMC: 2022 May 19.
Published in final edited form as: J Am Chem Soc. 2021 May 10;143(19):7380–7387. doi: 10.1021/jacs.1c00451

Figure 4.

Figure 4.

Folate-MS99 degrades ALK fusion proteins in a FOLR1-dependent manner. (A) Schematic illustration of the activation of folate-MS99 by endogenous hydrolase. (B) Western blot analysis of the NPM-ALK fusion protein level from SU-DHL-1 cells treated with indicated doses of MS99, folate-MS99, or folate-MS99N for 12 h. (C) Western blot analysis of NPM-ALK fusion protein from HT-29 cells after the cotreatment with free folic acid and 0.3 μM folate-MS99 for 12 h. (D) Western blot analysis of NPM-ALK fusion protein level in SU-DHL-1 cells after the cotreatment with 10 μM MG132 or 1 μM MLN4924 and 0.3 μM folate-MS99 for 12 h. (E) Cell viability of SU-DHL-1 cells after treatment with MS99, folate-MS99, or folate-MS99N for 72 h.