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. Author manuscript; available in PMC: 2021 Jul 20.
Published in final edited form as: Nature. 2021 Jan 20;590(7847):655–659. doi: 10.1038/s41586-020-03172-w

Figure 2. Primase is required to stabilize long leading strand ssDNA.

Figure 2.

a, Schematic of experiment in PRI2 (wt) and pri2–1 temperature-sensitive mutant. b, DNA synthesis in PRI2 (wt) (left) and pri2–1 (right) detected by AMBER. c, The extent of DNA synthesis in pri2–1 measured at 10hr as compared to wt. The means ± SD (n=3 independent biological repeats) are indicated. d, AMBER analysis of PRI2 (wt) and pri2–1 following treatment with S1-nuclease. b and d each represents one out of three independent biological repeats that showed similar results (see Supplementary Table 5 for other repeats). Mean values of target to reference (ACT1) loci ratios were calculated by Poisson distribution based on 10,000 droplets with error bars representing upper and lower Poisson 95%CI.