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. Author manuscript; available in PMC: 2021 Jul 20.
Published in final edited form as: Nature. 2021 Jan 20;590(7847):655–659. doi: 10.1038/s41586-020-03172-w

Figure 3. Interstitial telomere sequence (ITS) interrupts BIR.

Figure 3.

a, Human ITS inserted at 6 kb centromere-distal to site of strand invasion in the donor Chr III. Positions of primers before (0.2 kb and 4 kb centromere-distal from MATα-inc) and after (7.5 kb and 10 kb centromere-distal from MATα-inc) ITS are indicated. b, AMBER analysis using primers (indicated) in TLC1 (wt) and c, tlc1Δ. b and c each represents one out of three independent biological repeats that showed similar results (see Supplementary Table 5 for other repeats). Mean values of target to reference (ACT1) loci ratios were calculated by Poisson distribution based on 10,000 droplets with error bars representing upper and lower Poisson 95%CI. d, Examples of DSB repair outcomes analyzed by CHEF electrophoresis in TLC1 (left) and tlc1Δ (right). e, Fraction of GCRs identified similar to d, n=29 individual samples for TLC1 and n=21 individual samples for tlc1Δ from 3 independent biological repeats. (****=statistically significant difference (p=4e-6) by Chi-square test (two-sided, df=1)). f, Coverage of Illumina sequencing reads for GCR outcomes. Sites of MAT and ITS insertion are indicated. 2x-coverage peaks near 300kb position result from the presence of additional copies of the corresponding sequences in the genome.