Extended Data Figure 2. Kinetics of BIR synthesis.

a, Schematic of primers (triangles) used for AMBER analyses (similar to Fig. 1a). b, DNA synthesis detected by AMBER in a time course designed as described in Fig. 1b, but with nocodazole addition 3 hours prior to DSB induction (0hr). The data represent one experiment that was similar to 3 experiments presented in Fig. 2b. c, Slopes of BIR at the time when 50 percent of the cells had completed BIR (V₅₀) at the indicated chromosomal position based on three independent experiments (n=3), including the one shown in Fig. 1b. The means ± SD (error-bars) are indicated. Statistics is determined by two-tailed t-test with p value indicated. d, AMBER analysis of POL3 (wt), and e, pol3–01. b, d and e each represents one out of three independent biological repeats that showed similar results (see Supplementary Table 6 for other repeats). Mean values of target to reference (ACT1) loci ratios were calculated by Poisson distribution based on 10,000 droplets with error bars representing upper and lower Poisson 95%CI.