Figure 4. In situ pipeline.

Schematic representation of ZEBrA’s in situ hybridization pipeline. Serial cryostat brains sections encompassing most major brain structures are hybridized at close intervals (e.g. L1–8, in blue) for genes that exhibit highly differential patterns, or at select levels (e.g. L4/L6, in vermillion) for non-differential genes, alignment to reference sections from the histological atlas, and ZEBrA web publishing.