Author response image 2. Pharynx tissue loss selectively increases mitotically active pharynx progenitors.

(A) Proportion of FoxA⁺ H3P⁺ cells relative to all H3P⁺ stem cells at indicated times after pharynx or head amputation in the area outlined by dashed boxes in E. n ≥ 515 cells per experimental group from 2 independent experiments. (B) Average number of FoxA⁺ H3P⁺ cells quantified in the same animals and regions as A. Same data as is in Figure 3C, D of manuscript. (C) Proportion of cells double-positive for the indicated progenitor marker and H3P⁺ relative to all H3P⁺ stem cells in the area outlined by dashed boxes in E. n ≥ 472 cells per experimental group from 2 independent experiments. (D) Average number of cells double-positive for the indicated progenitor marker and H3P⁺ quantified in the same animals and regions as C. Same data as in Figure 3E of manuscript. (E) Cartoons depicting different amputation conditions. For A-D, the entire pre-pharyngeal region was analyzed over 30 z-sections, as represented by dashed boxes. Graphs represent a proportion ± 95% confidence intervals (A, C) or the mean ± SD with symbols = individual animals; shapes distinguish biological replicates (B, D). *, p ≤ 0.05 **, p ≤ 0.01; ***, p < 0.001; ****, p ≤ 0.0001, Fisher’s Exact Test (A, C) or one-way ANOVA with Tukey test (B, D)