Table 3.
Antigen test results for available clinical specimens containing D399N and T205I nucleocapsid mutants.
| Specimen | Cobas 6800 CT (E-gene) | Theoretical Roche cobas CT | Quidel Sofia 2 | Abbott BinaxNOW | Nucleocapsid Mutation | Lineage | GISAID accession |
|---|---|---|---|---|---|---|---|
| DM1 | 32.58 | 37.9 | Negative | Negative | T205I, D399N | B.1.429 | EPI_ISL_1209060 |
| DM2 | 21.85 | 27.2 | Negative | Positive | T205I, D399N | B.1.429 | EPI_ISL_1110097 |
| DM3 | 25.48 | 30.8 | Negative | Positive | T205I, D399N | B.1.429 | EPI_ISL_1789833 |
| DM4 | 20.44 | 25.8 | Negative | Positive | T205I, D399N | B.1.429 | EPI_ISL_1069365 |
| DM5 | 21.72 | 27.1 | Negative | Positive | T205I, D399N | B.1.429 | EPI_ISL_1366181 |
| DM6 | 23.25 | 28.6 | Negative | Positive | T205I, D399N | B.1.429 | EPI_ISL_1366195 |
| DM7* | 22.63 | 28.0 | Negative | n.d. | T205I, D399N | B.1.429 | EPI_ISL_1708574 |
| M8 | 15.29 | 20.7 | Positive | Positive | T205I | B.1.429 | EPI_ISL_1405087 |
| M9⁎⁎ | 24.27 | 29.6 | Positive | Negative | T205I | B.1.429 | EPI_ISL_1405089 |
| M10⁎⁎ | 22.3 | 27.6 | Negative | Positive | T205I | B.1.429 | EPI_ISL_1405090 |
| M11 | 21.21 | 26.6 | Positive | Positive | T205I | B.1.429 | EPI_ISL_1405112 |
| M12 | 24.41 | 29.7 | Positive | Positive | T205I | B.1.429 | EPI_ISL_1405113 |
| M13 | 22.47 | 27.8 | Positive | Positive | T205I | B.1.429 | EPI_ISL_1405115 |
| M14 | 24.06 | 29.4 | Positive | Positive | T205I | B.1.429 | EPI_ISL_1405119 |
| M15 | 23.4 | 28.7 | Positive | Positive | T205I | B.1.429 | EPI_ISL_1405138 |
| M16 | 20.06 | 25.4 | Positive | Positive | T205I | B.1.429 | EPI_ISL_1405141 |
| M17 | 22.78 | 28.1 | Positive | Positive | T205I | B.1.429 | EPI_ISL_1405148 |
| M18 | 17.47 | 22.8 | Positive | Positive | T205I | B.1.429 | EPI_ISL_1405151 |
⁎
Original discordant sample.
⁎⁎
M9 and M10 were diluted to create enough volume to run on cobas 6800 for a fresh CT (Dilution was prepared prior to both antigen and molecular testing)
n.d., not done.