Table 4.
Antigen testing results for detection of transiently expressed nucleocapsid proteins. The approximate initial limit of detection is highlighted in bold, where both highlighted 2/3 decisions involved a split decision on the 3rd specimen in question. The estimated amount of protein placed on a swab was calculated based on the western blot in Fig. 4.
| Sample | Dilution | Sofia 2 | BinaxNOW | QuickVue | Estimated protein (pg) |
|---|---|---|---|---|---|
| Negative Control | 1:100 | 0/1 | 0/1 | 0/3 | |
| Wild-type N | 1:10 | 3/3 | 3/3 | 560,400 | |
| 1:100 | 3/3 | 3/3 | 56,040 | ||
| 1:1000 | 3/3 | 3/3 | 3/3 | 5604 | |
| 1:10,000 | 3/3 | 3/3 | 3/3 | 560 | |
| 1:100,000 | 0/3 | 3/3 | 2/3 | 56 | |
| 1:1,000,000 | 0/3 | 0/3 | 6 | ||
| N T205I | 1:10 | 3/3 | 1/1 | 1/1 | 948,500 |
| 1:100 | 3/3 | 1/1 | 3/3 | 94,850 | |
| 1:1000 | 3/3 | 3/3 | 3/3 | 9485 | |
| 1:10,000 | 3/3 | 3/3 | 3/3 | 948 | |
| 1:100,000 | 0/3 | 2/3 | 3/3 | 95 | |
| 1:1,000,000 | 0/3 | 0/3 | 9 | ||
| N D399N | 1:10 | 6/6 | 3/3 | 673,200 | |
| 1:100 | 1/6 | 3/3 | 67,320 | ||
| 1:1000 | 0/3 | 3/3 | 3/3 | 6732 | |
| 1:10,000 | 0/3 | 3/3 | 3/3 | 673 | |
| 1:100,000 | 0/3 | 3/3 | 2/3 | 67 | |
| 1:1,000,000 | 0/3 | 0/3 | 7 | ||
| N T205I/D399N | 1:10 | 4/4 | 1/1 | 1/1 | 1,220,800 |
| 1:100 | 2/6 | 1/1 | 3/3 | 122,100 | |
| 1:1000 | 0/3 | 3/3 | 3/3 | 12,210 | |
| 1:10,000 | 0/3 | 3/3 | 3/3 | 1221 | |
| 1:100,000 | 0/3 | 3/3 | 3/3 | 122 | |
| 1:1,000,000 | 0/3 | 0/3 | 12 |