Fig. 1. S1P2 antagonist reduced atherosclerosis in Apoe^－/－ mice .

S1P2 expression increased in Apoe ^－/－ mice fed with WTD compared with wild-type mice fed with normal chow. The expression of S1P1 and S1P3 was higher in Apoe ^－/－ mice compared with wild-type mice; however, it did not reach statistical significance. (B) En-face Sudan IV staining showed that S1P2 antagonist administration for 20 weeks to Apoe ^－/－ mice significantly decreased the development of atherosclerosis in the aortic arch ( n = 13–15, per group). (C) qPCR demonstrated that administration of S1P2 antagonist for 20 weeks decreased the expression of inflammatory molecules in atherosclerotic thoracic aorta compared with vehicle ( n = 13–15, per group). Bar, 1 mm. ^＊ ; p ＜0.05. All values are mean±SEM.