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. 2021 Jun 15;27:101044. doi: 10.1016/j.bbrep.2021.101044

Fig. 3.

Fig. 3

NEM induces distinct TRPA1 activation phases in cell-attached single-channel recordings. A and B, Representative open probability (NPo) analysis of single TRPA1 channels induced by NEM (30 μM) into partial (A) and full (B) activation phases. P1/F1 denotes prior to activation, P2/F2 denotes phase following initiation of activation, F3 denotes full activation phase. C, maximum and mean NPo analysis for full (F, n = 13) and partial (P, n = 10) TRPA1 activation recordings and non-transfected HEK293 (Nt, n = 5). #, p < 0.05 vs non-transfected; *, p < 0.05 vs full. D and E, amplitude histograms for partial (D) and full (E) activation shown in A and B. F, mean ± S.E.M. current for channels induced into partial and full (separated into paired F2 and F3 phases) activation. G and H, histogram of open time distribution for partial (G) and full (H) activation shown in A and B; fitted by the sum of exponentials. I and J, mean ± S.E.M. of open time exponential τ (I) and proportion (J) for partial and full activation channels. *, p < 0.05 vs partial. K and L, histogram of closed time distribution for partial (K) and full (L) activation shown in A and B; fitted by the sum of exponentials. M and N, mean ± S.E.M. of closed time exponential τ (M) and proportion (N) for partial and full activation channels. *, p < 0.05 vs partial. O, mean ± S.E.M. time to threshold for partial and full activation. P, Linear regression comparing the time to threshold vs. Δ time to full activation (p > 0.05). Q, mean NPo for all WT (n = 31) and C665S (n = 19) recordings during NEM treatment (30 μM). R, mean ± S.E.M. current for WT and C665S channels. S, percentage of TRPA1 channels that reach full activation for WT (13/49) and C665S (3/44) ($, p < 0.05). T, mean ± S.E.M. time to threshold for all individually-recorded WT (n = 23) and C665S (n = 12) channels. U-X, mean ± S.E.M. of open (U, V) and closed time (W, X) exponential τ (U, W) and proportion (V, X) for partial activation (P2) in WT and C665S channels. $, p < 0.05 vs WT. Y, mean NPo analysis for partial activation (P2) in WT and C665S channels. *, p < 0.05 vs baseline (paired); $, p < 0.05 vs WT (unpaired).