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. 2021 Jun 22;11:13025. doi: 10.1038/s41598-021-92508-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Summary of pathways analysis using MetaboAnalyst (https://www.metaboanalyst.ca): (1) phenylalanine, tyrosine and tryptophan biosynthesis; (2) pyruvate metabolism. (B) Detailed results of pathway analysis. Total Cmpd is the total number of compounds in the pathway; Hits is the actually matched number of compounds from the user uploaded data; Raw p is the original p value calculated from the enrichment analysis; Holm is the p value adjusted by Holm-Bonferroni method; FDR is the p value adjusted using False Discovery Rate; Impact is the pathway impact value calculated from pathway topology analysis (EPIC Florence, low- vs high-MBD BC case–case study).