. 2021 Jun 23;26(1):633–642. doi: 10.1007/s00784-021-04041-0

Table 2.

Optical density of Streptococcus mutans suspensions according to the treatment and drug concentration

	Treatment		Chlorhex	Chlorhex/30 min/nystatin	Nystatin/30 min/chlorhex	Chlorhex/1 min/nystatin	Nystatin/2 min/chlorhex	Chlorhex/10 min/nystatin	Nystatin/10 min/chlorhex	Chlorhex/nystatin single	P*
Optical density (at 600 nm)	C1	Mean	0.155^B	1.223^A	0.171^B	1.354^A	1.556^A	1.242^A	0.305^C	0.102^D	0.000
	C1	SD	0.019	0.197	0.039	0.189	0.233	0.324	0.072	0.009	0.000
	C2	Mean	0.090^A	0.171^B	0.146^D	1.069^C	0.297^BE	0.231 ^E	0.152^BD	0.178^ABDE	0.000
	C2	SD	0.003	0.011	0.015	0.234	0.094	0.026	0.011	0.079	0.000
	C3	Mean	0.099^A	0.152^B	0.134^BD	0.111^C	0.132^BD	0.137^BD	0.134^BD	0.111^ACD	0.000
	C3	SD	0.003	0.025	0.008	0.003	0.009	0.007	0.005	0.017	0.000
	C4	Mean	0.060^A	0.059^A	0.059^AB	0.056^B	0.057^AB	0.060^ABC	0.059^A	0.050^C	0.000
	C4	SD	0.002	0.001	0.003	0.001	0.002	0.008	0.001	0.002	0.000
	C5	Mean	0.053^A	0.055^AB	0.053^AB	0.055^AB	0.053^AB	0.054^AB	0.051^B	0.049^AB	0.003
	C5	SD	0.001	0.003	0.003	0.003	0.003	0.004	0.001	0.002	0.003
	C6	Mean	0.053^A	0.057 ^AB	0.053^AB	0.056^AB	0.053^AB	0.055^A	0.054^AB	0.049^B	0.012
	C6	SD	0.001	0.005	0.003	0.007	0.003	0.003	0.004	0.002	0.012
	C7	Mean	0.053^A	0.052^AB	0.052^AB	0.053^AB	0.052^AB	0.051^AB	0.051^AB	0.050^B	0.036
	C7	SD	0.002	0.001	0.002	0.002	0.001	0.002	0.002	0.003	0.036
	C8	Mean	0.053	0.056	0.057	0.055	0.054	0.054	0.053	0.054	0.498
	C8	SD	0.001	0.002	0.007	0.002	0.003	0.003	0.002	0.006	0.498
	Negative control	Mean	0.926	0.432	0.533	0.197	0.424	0.198	0.207	0.215
	Negative control	SD	0.124	0.285	0.308	0.021	0.245	0.040	0.036	0.073

C1 64 μg/mL chlorhexidine and 32 μg/mL nystatin, C2 32 μg/mL chlorhexidine and 16 μg/mL nystatin, C3 16 μg/mL chlorhexidine and 8 μg/mL nystatin, C4 8 μg/mL chlorhexidine and 4 μg/mL nystatin, C5 4 μg/mL chlorhexidine and 2 μg/mL nystatin, C6 2 μg/mL chlorhexidine and 1 μg/mL nystatin, C7 1 μg/mL chlorhexidine and 0.5 μg/mL nystatin, C8 0.5 μg/mL chlorhexidine and 0.25 μg/mL nystatin. Chlorhex chlorhexidine, SD standard deviation

^*P value for ANOVA comparing the treatments within each concentration; means followed by different letters in the same row differ significantly (α = 0.05) by Dunnett’s T3 multiple comparisons test

Negative control medium and Streptococcus mutans culture without drugs; not included in the statistical analysis