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. 2021 Jun 22;11:13075. doi: 10.1038/s41598-021-92080-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of GA₃ treatment on fruit color and estimated anthocyanin content at ripeness in early- and mid-season varieties (55 and 64 DAFB, respectively) in the 2017–2018 season. GA₃ was applied as the commercial product ProGibb 40% SG to individual branches at a rate of 30 ppm. GA₃ treatment was at the light green-to-straw yellow transition of the fruits at 35 DAFB in both varieties (November 2, 2017). (A,B) Color distribution according to CTIFL color chart (1 is the lightest and 4 is darkest fruit color detected). The percentage (%) is calculated considering the number of fruits having a given category over the total number of fruits. (C,D) Representative picture of control- and GA₃-treated fruits. (E,F) Endogenous estimated anthocyanins in the fruits on a fresh weight (FW) basis, based on cyanidin-3-O-rutinoside content, representing more than 98% of the total anthocyanins in each of the samples. Bars represent the mean of three independent biological replicates ± SEM. The significance of variation between control- and GA₃-treated fruits was tested by one-way ANOVA analysis with Tukey's post hoc test, whereby different letters are significantly different means (p < 0.05). Graphs were performed on GraphPad Prism version 6.04 for Windows, GraphPad Software, www.graphpad.com.