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. 2021 Jun 22;11:13084. doi: 10.1038/s41598-021-91086-6

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© The Author(s) 2021, corrected publication 2021, 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Chemical disruption of the hTRiC 16-mer enables subsequent dimerization. (a) The monomer signal intensity of the same hTRiC sample increases over 100-fold upon addition of 25% DMSO or methanol (MeOH) (NL = Normalized Level). This supports the original presence of predominantly intact 16-mers and their thorough dissociation into monomers. (b) Native mass spectra of intact and solution-dissociated hTRiC. Monomer, dimer, and 16-mer regions of signal are observed. (c) Detail view of the dimer regions in b. Peaks are sufficiently resolved to assign dimers unambiguously; three charge states for the five most abundant assigned dimers are annotated in each mass spectrum. (d) Relative abundances of all assigned CCT dimers after DMSO or methanol dissociation. Squares with a bold outline represent interfaces that exist in correctly assembled TRiC. The underlying data and quantification of each subunit’s contribution to the total dimer intensity is provided in Supplementary Data 4. (e) Schematic of TRiC assembly and dissociation in vitro and in vivo, where CCT5 is posited to play a key role in processes involving dimers.