Table 1.

Design scheme of E. coli nonsense tRNA suppressors.

Properties	Sequence design
Secondary structure	(((((((..((((……..)))).(((((…….)))))…..(((((…….))))))))))))….
Tertiary interactions	…….UAGCUCAG..GG.AGAGC………………G.G.C…..UUCGAUU…………….
AlaRS identity	GGGG……………G…………………………………………CUCCACCA
New anticodon	……………………………CUA………………………………….
Combined restrains	GGGG…UAGCUCAG..GGGAGAGC……..CUA…….G.G.C…..UUCGAUU……..CUCCACCA

Secondary structure and major restraints in the design are shown. Bold bases were considered in the designs t1 and t2 only.