FIGURE 4.

MYOF silencing reduces the malignant phenotype of 5-8F cells. (A) Cellular proliferation of control and MYOF knockdown 5-8F cells when passaged for the indicated days. Independent experiments performed in triplicate (**p < 0.01 and ***p < 0.001, respectively, Students t-test). (B) Wound-healing assay performed with Vector- or shMYOF-transfected 5-8F cells. Representative images acquired at the indicated time points are shown. The unhealed area was measured in three independent experiments (**p-value < 0.01). (C) Control or MYOF knockdown 5-8F cells were subjected to Transwell migration assays in three independent experiments. Migratory cells were counted under a microscope (**p < 0.01). (D) Control or MYOF knockdown 5-8F cells were subjected to Transwell invasion assays (with Matrigel). Invasive cells were counted under a microscope (**p < 0.01).