TABLE 2.
Top 20 list of up- and downregulated proteins in 5-8F vs. 6-10B cells.
| Symbol | Protein name | Score | Peptides | Log2(MR) | P-value | PM | CS | SR | EC |
| Up-regulated proteins | |||||||||
| MYOF | Myoferlin | 323.31 | 146 | 4.54 | 1.50E–06 | + | + | ||
| ATP1A1 | Sodium/potassium-transporting ATPase subunit alpha-1 | 323.31 | 62 | 4.22 | 7.34E–05 | + | + | ||
| ABCC1 | Multidrug resistance-associated protein 1 | 266.82 | 37 | 3.78 | 1.74E–03 | + | + | ||
| SLC16A1 | Monocarboxylate transporter 1 | 206.15 | 4 | 3.74 | 1.39E–03 | + | + | ||
| HMOX2 | Heme oxygenase 2 | 323.31 | 20 | 3.72 | 1.09E–02 | + | |||
| RTN4 | Reticulon-4 | 285.14 | 15 | 3.65 | 8.20E–04 | + | + | ||
| ATP2B1 | Plasma membrane calcium-transporting ATPase 1 | 323.31 | 53 | 3.59 | 2.08E–06 | + | + | ||
| ATP2B4 | Plasma membrane calcium-transporting ATPase 4 | 323.31 | 24 | 3.55 | 1.02E–05 | + | |||
| SLC7A5 | Large neutral amino acids transporter small subunit 1 | 247.22 | 7 | 3.49 | 3.11E–04 | + | + | ||
| SLC1A5 | Neutral amino acid transporter B(0) | 284.44 | 15 | 3.34 | 7.46E–05 | + | + | ||
| ATP1B3 | Sodium/potassium-transporting ATPase subunit beta-3 | 107.89 | 14 | 3.33 | 3.99E–03 | + | + | ||
| PON2 | Serum paraoxonase/arylesterase 2 | 86.501 | 3 | 3.29 | 3.36E–05 | + | + | ||
| HTRA2 | Serine protease HTRA2 | 26.469 | 10 | 3.28 | 1.18E–02 | + | |||
| GNA13 | Guanine nucleotide-binding protein subunit alpha-13 | 26.947 | 11 | 3.26 | 3.27E–02 | + | + | ||
| EGFR | Epidermal growth factor receptor | 323.31 | 49 | 3.23 | 3.04E–03 | + | + | + | + |
| PVR | Poliovirus receptor | 165.18 | 8 | 3.17 | 4.38E–03 | + | + | + | |
| SLC2A1 | Solute carrier family 2 | 57.723 | 6 | 3.15 | 1.50E–04 | + | + | ||
| IGF1R | Tyrosine-protein kinase receptor | 17.675 | 11 | 3.1 | 1.14E–02 | + | + | ||
| GOT2 | Aspartate aminotransferase | 300.24 | 20 | 3.05 | 7.17E–03 | + | + | ||
| ICAM1 | Intercellular adhesion molecule 1 | 226.69 | 19 | 3.02 | 5.84E–05 | + | + | + | |
| Down-regulated proteins | |||||||||
| MYO1C | Unconventional myosin-Ic | 323.31 | 64 | −4.73 | 1.55E–03 | + | + | ||
| MYO6 | Unconventional myosin-VI | 323.31 | 61 | −4.42 | 6.56E–03 | + | + | ||
| PHB2 | Prohibitin-2 | 323.31 | 29 | −4.42 | 1.85E–05 | + | + | ||
| PHB | Prohibitin | 323.31 | 27 | −4.25 | 8.51E–05 | + | + | + | |
| CKAP4 | Cytoskeleton-associated protein 4 | 323.31 | 45 | −3.99 | 3.06E–06 | + | + | ||
| SEPT | Septin-7 | 203.21 | 17 | −3.59 | 1.60E–02 | + | + | ||
| SEPT2 | Septin-2 | 323.31 | 19 | −3.43 | 2.39E–05 | + | + | ||
| MYO1B | Unconventional myosin-Ib | 323.31 | 41 | −3.15 | 1.73E–03 | + | + | ||
| LIMA1 | LIM domain and actin-binding protein 1 | 323.31 | 61 | −3.05 | 2.04E–06 | + | |||
| ABCG2 | ATP-binding cassette sub-family G member 2 | 41.72 | 9 | −2.93 | 2.07E–02 | + | |||
| CORO1C | Coronin-1C | 167.2 | 25 | −2.76 | 7.03E–05 | + | |||
| IQGAP1 | Ras GTPase-activating-like protein IQGAP1 | 323.31 | 102 | −2.69 | 1.16E–04 | + | + | ||
| KTN1 | Kinectin | 323.31 | 70 | −2.59 | 8.49E–04 | + | |||
| MAP4 | Microtubule-associated protein | 179.13 | 18 | −2.43 | 1.39E–02 | + | + | ||
| ALPP | Alkaline phosphatase | 323.31 | 23 | −2.42 | 1.45E–05 | + | + | ||
| SLC25A11 | Mitochondrial 2-oxoglutarate/malate carrier protein | 111.51 | 12 | −2.37 | 1.29E–04 | + | |||
| SERBP1 | Plasminogen activator inhibitor 1 RNA-binding protein | 202.11 | 9 | −2.25 | 9.35E–03 | + | + | ||
| LMO7 | LIM domain only protein 7 | 323.31 | 44 | −2.16 | 1.15E–04 | + | |||
| MAP7 | Ensconsin | 21.18 | 10 | −2.11 | 2.70E–02 | + | |||
| CAPRIN1 | Caprin-1 | 106.87 | 9 | −2.1 | 6.40E–03 | + | |||
The listed proteins were analyzed with multiple biological repeats (heavy high metastatic NPC cell line: light low metastatic NPC cell line). For each protein, the following information is shown: adjusted P-values, logarithm of median ratio (high/low), number of quantified razor peptides plus unique peptides in a replicate. For identification, the peptide and protein FDRs were both set to 1% when using MAXQUANT. Peptides, Razor + unique peptides; MR, median ratio; PM, plasma membrane; CS, cell surface; SR, signaling receptor; EC, extracellular.