Fig. 1.

IFNγ induces morphological changes in breast cancer cells and modulates their transcriptome.

A) The morphology of MDA-MB-231 and MCF-7 cells changed after IFNγ treatment for 24 h. Cells were stained with calcein. Magnified view of a representative cell marked in blue, indicating the quantified diameter. B) Cell diameters were quantified to demonstrate the morphological changes in breast cancer cells in response to IFNγ. C) Heat maps of IFNγ-regulated genes in both breast cancer cells. D) Heat maps of the commonly regulated genes in both MCF-7 and MDA-MB-231 cells by IFNγ. IFNγ down- and upregulated genes are displayed in red and green color, respectively. E) Venn diagram showing the percentage of genes regulated in both breast cancer cell lines.