Lysis buffer (for mammalian cells, 50 mL)
| Reagent | Final concentration | Add |
|---|---|---|
| 25% Sarkosyl | 1% | 2 mL |
| 0.5 M EDTA | 0.475 M | 47.5 mL |
| Proteinase K | 200 mg/mL | 500 μL |
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