Table 2.
Characteristics of lesions and result of diagnosis of 12 patients of CL.
| Patient's Id | Smear | rK39 | Culture | Leishmania species -specific identification by PCR- RFLP | Leishmania species -specific identification by sequencing method | Type,size and site of lesion | Village/block/district |
|---|---|---|---|---|---|---|---|
| 1 | Negative | Positive | Negative | L. donovani | L. donovani | An well-defined Single erythematous plaque over the dorsum of nose and adjacent cheeks of size 15 cm × 4 cm approx., yellowish crusting over surface, discharge of pus surrounding erythematous border. | Chagon/Nichar/kinnuar |
| 2 | Negative | Negative | Negative | L. donovani | L. donovani | An well-defined Single shining plaque on left cheek near the ear of size 1.5 × 1cm approx. | Anni/Anni/Kullu |
| 3 | Positive | Negative | Negative | L. donovani | L. donovani | An well-defined shining plaque over the left cheek of size 3 × 2cm approx. | Jagaat khaana/Nirmand/Kullu |
| 4 | Negative | Negative | Negative | L. major | L. major | A single well defined nodular plaque over the dorsum of nose of size 3 × 3cm approx. | Gasso/Rampur/Shimla |
| 5 | Negative | Negative | Negative | L. major | L. major | A single well defined plaque with two satellite lesion in vicinity on ear lobule of size 3 × 1.5cm approx. | Chaura/Nichar/Kinnaur |
| 6 | Negative | Negative | Negative | L. donovani | L. donovani | A single well defined erythematous plaque of size 1.5 × 1.5cm approx. on right forearm with central crusting. | Anni/Kullu |
| 7 | Negative | Negative | Negative | L. tropica | L. tropica | A single well defined ulcerated plaque with damage over lower and upper lip of size 2 × 2cm approx. | Yangpa/Nichar/Kinnaur |
| 8 | Negative | Negative | Negative | L. donovani | L. donovani | An well-defined single shining papule with no secondary changes over left cheek of size 4 × 4cm approx. | Randal/Brow/Kullu |
| 9 | Negative | Negative | Negative | Not identified | Sequences not readable | A single well defined crusted plaque present below left eye of size 1 × 1cm approx. | Jagaat khaana/Nirmand/Kullu |
| 10 | Negative | Negative | Negative | L. tropica | L. tropica | An well-defined shining plaque over left upper lip of size 4 × 4 cm approx. | Ranjan/theog/Shimla |
| 11 | Negative | Negative | Negative | L. donovani | L. donovani | An well Ulcerated plaque over distil pharynx of middle finger of size 2 × 2cm approx. | Brow/Nirmand/kullu |
| 12 | Negative | Negative | Negative | L. tropica | L. tropica | An well defined erythematous nodule plaque of size 3 × 3cm over the tip of the nose, adjacent cheeks and on wrist. | Kimcha/Kushwa/Kullu |