FIGURE 4.

Levobupivacaine-upregulated miR-489-3p enhances ferroptosis of gastric cancer cells. (A) HGC27 and SGC7901 cells were treated with saline or levobupivacaine (2 mM). The qPCR analysis of miR-489-3p expression. (B,C) HGC27 and SGC7901 cells were co-treated with erastin (5 μM) and miR-489-3p mimic. MTT assays for cell viability analysis. (D–I) The erastin (5 μM) and RSL3 (1 μM)-stimulated HGC27 and SGC7901 cells were treated with miR-489-3p mimic. The Fe²⁺ (D,E), iron (F,G), and ROS levels (H,I) were analyzed. The experiments were performed independently three times (mean ± SD, **p < 0.01).