Figure 1.

GrK inhibits angiogenesis in vitro. A co-culture of pericytes and endothelial cells in a 3D collagen gel was treated with a concentration series of GrK and GrKSA. (A) Representative fluorescent microscope images of GFP-labeled HUVECs (green) in co-culture with dsRED-labeled pericytes (red) in a 3D collagen matrix during vascular formation. Shown are the results at day 3 of an untreated control and after stimulation with different concentrations of GrK or GrKSA. Scale bar =500µm. Right corner corresponding to the inset, scale bar=100µm. Angiogenesis was described via (B) number of junctions, (C) number of tubules, (D) mean tubule length and (E) total tubule length. For all parameters, relative values compared to mock concentration (100%) are shown. Results are depicted as mean ± SEM, n≥10. *P < 0.05; **P < 0.01; ***P < 0.001, compared to control.